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10/05/2018

The synthesis of artificial cellular microreactors on the front page of Molecular Systems Biology scientific journal

This month, the scientific journal Molecular Systems Biology dedicates the front page of its latest issue to an article on the synthesis of artificial cellular microreactors by François Fages, head of the Lifeware research team, Alexis Courbet (CNRS/INSERM), Patrick Amar (CNRS/LRI), Eric Renard (INSERM/CNRS) and Franck Molina (CNRS).

Computer-aided biochemical programming of synthetic microreactors as diagnostic devices

Synopsis :

 

 

A systematic approach is presented to design and encapsulate biochemical logic circuits within synthetic phospholipid bilayers that operate as synthetic microreactors. As proof‐of‐concept, such devices were programmed to detect specific patterns of metabolic biomarkers for the diagnosis of diabetes.

  • We introduce the first complete workflow based on computational design and microfluidics for the programming of synthetic cell‐like microreactors using biochemical logic circuits, to perform biosensing and biocomputing operations in vitro .
  • For the first time we show that the implementation of Boolean logic circuits with reactive biochemical species can be automated to satisfy user defined temporal logic specifications and their behavior optimized for robustness.
  • We demonstrate the programming, synthesis and operability of three different instances of synthetic biochemical logic circuits encapsulated within synthetic phospholipid bilayers.
  • Using these methodologies, we generate proof‐of‐concept diagnostic microreactors, or protosensors, biochemically programmed to detect specific patterns of biomarkers and classify pathological states in situ . We demonstrate their capabilities for the diagnosis of acute diabetes complications in human clinical samples.

L'équipe de recherche Lifeware
Lifeware est une équipe-projet Inria, située au centre de recherche Inria Saclay - Île de France (anciennement Paris-Rocquencourt jusqu'en janvier 2016) sur le campus de l'École polytechnique de Palaiseau. Créé en janvier 2014, dans le cadre du suivi de l'équipe-projet Contraintes, Lifeware vise à développer des méthodes formelles et des cadres expérimentaux pour comprendre la machinerie cellulaire et établir des paradigmes de calcul en biologie cellulaire. Elle s'appuie sur la vision des cellules comme machines, des réseaux de réaction biochimique comme programmes, et sur l'utilisation de concepts et d'outils issus de l'informatique pour maîtriser la complexité des processus cellulaires.

Keywords: François Fages Méthodes formelles Inria Saclay - Île-de-France Lifeware Biologie cellulaire

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